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Ligation may refer to:

• The act of making a ligature (tie). In medicine, a ligature is a device, similar to a tourniquet, usually of thread or string, tied around a limb, blood vessel or similar to restrict blood flow. A ligature can be used for tumor strangulation, carried out before removal.
• Chemical ligation, the process of producing peptides from amino acids
• The DNA-ligating action of DNA ligase
• Tubal ligation, a method of female sterilization

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